192 Wistar male rats (180- 200 g) were used to evaluate the prophylactic effect of ozone combined with antibiotics in septic shock model. The proinflammatory cytokines (IL-1b, TNFa, IL-6 and IL-2) expression were also determinated in different haemathopoyetic organs. The animals were taken at random and divided into 16 groups, 12 animals each: Group A, positive control (sepsis without treatment); groups B and C treated with ozone (200 ug / 250 g b. w) and Cefotaxim (25mg/kg), (0h, +1h) and (+1 h) respectivitly; groups D and E, ozone and Cefodizim (25mg/kg (0h, +1h) and (+1 h) respectively; groups F and G ozone and levofloxacin (12.5 mg/kg), (0h, +1h) and (+1 h) respectively; groups H and I ozone and Piperacillin /Tazobactam (65mg/kg), (0h, +1h) and (+1 h) respectively. The septic shock was carried out by single intraperitoneal injection of fecal material (0.65 g/kg b/w) from a donor rat, diluted in saline solution 0.9%. The other groups (J-P) of animals were treated only with antibiotics.

The group pretreated with ozone and antibiotics showed a significant increase in the survival rate and showed also a decrease of IL-1b mRNA expression in liver. Ozone pretreatment might prevent lethal peritonitis by controlling inflammatory process generated physiologically into the body against specific damage or specific organism.